Sequencing
Next-generation sequencing, start to finish.
Short- and long-read sequencing for genomes, exomes, targeted regions and transcriptomes. We handle extraction, library preparation, sequencing and primary analysis, and hand back analysis-ready data with QC you can inspect.
Assays
Choose the resolution your question needs
Depth and read length are matched to the biology — we will recommend the most efficient option rather than the largest.
Whole-genome sequencing (WGS)
The most complete view of a genome: SNVs, indels, copy-number and structural variants, and non-coding regions. PCR-free libraries available for the cleanest coverage.
- Typical depth
- 30× (human) · custom
- Read mode
- 2 × 150 bp
- Best for
- Discovery, rare variants, SV
Whole-exome sequencing (WES)
Deep coverage of protein-coding regions at a fraction of WGS cost — a practical default for variant discovery in coding sequence. Capture kits selected to your target.
- Typical depth
- 100× on-target
- Read mode
- 2 × 150 bp
- Best for
- Coding variants, cohorts
Targeted & custom panels
Amplicon or hybrid-capture panels — catalogue or designed to your gene list — sequenced deep for sensitive detection of low-frequency variants.
- Typical depth
- 500–1000×+
- Design
- Custom or catalogue
- Best for
- Focused, sensitive assays
RNA sequencing
Gene expression, differential expression, splicing and fusion detection. polyA or total-RNA (rRNA-depleted), stranded libraries for accurate strand assignment.
- Typical depth
- 20–50M reads
- Library
- polyA / total RNA
- Best for
- Expression, splicing, fusions
Low-pass WGS
Sparse genome-wide sequencing followed by imputation to a reference panel — millions of genotypes at low cost. The backbone of our agrigenomics work.
- Typical depth
- 0.5–2×
- Readout
- Imputed genotypes
- Best for
- Genotyping at scale
Long-read sequencing
Oxford Nanopore for large structural variants, phasing, repeat expansions, native base modifications and full-length transcript isoforms — alone or paired with short reads.
- Platform
- Oxford Nanopore
- Strengths
- SV, phasing, methylation
- Best for
- Hard regions, isoforms
Platforms
Short- and long-read, matched to the job
We are platform-agnostic. Where a project benefits from combining technologies — for example short-read accuracy with long-read structural resolution — we run both and integrate the results.
Sample requirements
What to send
Indicative starting material. We routinely work with limited and degraded samples — ask us about low-input and FFPE options before you assume a sample is unusable.
| Sample | Typical input | Notes |
|---|---|---|
| Genomic DNA | ≥ 200 ng–1 µg | Low-input protocols available |
| Total RNA | ≥ 100 ng, RIN ≥ 7 | DNase-treated preferred |
| FFPE | Case by case | QC-gated; we advise first |
| Cells / tissue / blood | By arrangement | We can extract for you |
Deliverables
What you receive
- Raw reads (FASTQ), demultiplexed per sample.
- Alignments (BAM / CRAM) to your chosen reference, on request.
- Variant calls (VCF) and reports when analysis is included — see bioinformatics.
- A QC report: yield, quality, duplication, coverage uniformity.
- Pipeline names, versions and parameters used.
- Secure transfer; raw data archived so the study can be re-analysed later.
Questions
Sequencing FAQ
Can you extract DNA or RNA from my samples?
Yes. Send tissue, blood, cells or other material and we will handle extraction and QC, or you can ship purified nucleic acid to our specifications.
Which reference genome do you align to?
Your choice — for human work we default to GRCh38, and we support non-human and custom references. We state the exact build and annotation in the delivery.
Do I get the raw data, or only a report?
Always the raw data. You own it. Reports and processed files are in addition to FASTQ, never a replacement for it.
Can you combine short and long reads on the same sample?
Yes — hybrid analysis improves structural-variant and copy-number resolution. We will tell you whether it is worth the added cost for your specific question.
Have samples ready?
Tell us the sample type, how many, and what you want to learn. We will reply with a recommended assay, depth, timeline and price.